zf-H2C2_2; Zinc-finger double domain; NM_016260.3 → NP_057344.2 zinc finger protein Helios isoform 1. See identical proteins and their annotated locations for NP_057344.2. Status: REVIEWED. Description Transcript Variant: This variant (1) represents the shorter transcript but encodes the longer isoform (1). Source sequence(s)

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Apr 12, 2014 - Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.

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Zinc fingers perform many different jobs, as shown in these three sample structures. The complex at upper left (PDB entry 1y0j ) shows zinc fingers from two longer proteins, GATA-1 (which contains two zinc fingers) and FOG-1 (which contains 9 zinc fingers). The specific interaction between these two zinc fingers plays an essential role in the ...

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A zinc finger is a small protein structural motif that is characterized by the coordination of one or more zinc ions (Zn 2+) in order to stabilize the fold.It was originally coined to describe the finger-like appearance of a hypothesized structure from the African clawed frog (Xenopus laevis) transcription factor IIIA.However, it has been found to encompass a wide variety of differing protein ...

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Engineered zinc finger nucleases (ZFNs) enable the rapid introduction of targeted mutations in zebrafish. ZFNs consist of an engineered zinc finger array, typically composed of three or four fingers, fused to the non-specific cleavage domain from the Type IIS restriction enzyme FokI ().ZFNs function as dimers, with each monomer binding to "half-sites" separated by a spacer …

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Addgene depositor Charles Gersbach used paired zinc finger nucleases to remove exon 51 in DMD patient myoblasts. They observed a 13% removal rate of exon 51, which resulted in appropriately localized dystrophin. In a subsequent study, ...

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Plasmid pSG5-FLAG-mEKLF Zinc Fingers from Dr. James Bieker's lab contains the insert EKLF and is published in Mol Cell Biol. 2001 May;21(9):3118-25. This plasmid is available through Addgene.

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ZiFiT (Zinc Finger Targeter) is a simple and intuitive web-based tool that provides an interface to identify potential binding sites for engineered zinc finger proteins (ZFPs) in user-supplied DNA sequences. In this updated version, ZiFiT identifies potential ...

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pRS-ZFP: Three-zinc finger protein specifically targeting a 9-bp BCR-ABL sequence was amplified by PCR from plasmid pGP-FB (Addgene, Cambridge MA, the U.S.) using a forward primer ZFF(5'-CCCACTAGTACAATCAACTCC ATGGGACC-3') with a SpeI site and a reverse primer (5'-CCCGAATTCCCT CAGGTGGGTTTTTAGG-3') with an EcoRI site and cloned into pRS-ADH to ...

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Zinc finger proteins are among the most abundant proteins in eukaryotic genomes. Their functions are extraordinarily diverse and include DNA recognition, RNA packaging, transcriptional activation, regulation of apoptosis, protein folding and assembly, and lipid binding. Zinc finger structures are as …

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XM_005250181.4 → XP_005250238.1 zinc finger protein 800 isoform X3 See identical proteins and their annotated locations for XP_005250238.1 UniProtKB/Swiss-Prot

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zf-C2H2; Zinc finger, C2H2 type pfam13465 Location: 389 → 414 zf-H2C2_2; Zinc-finger double domain; NM_001324347.2 → NP_001311276.1 zinc finger protein 239 isoform a. Status: VALIDATED. Description Transcript Variant: This variant (5), as well as variants 1-4 and 6-9, encodes isoform a. Source sequence(s) AL450326 Consensus CDS CCDS41502.1 ...

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help@addgene Zinc Finger Consortium Expression Vector and Modular Assembly Kit Description: The Zinc Finger Consortium () was established to promote continued research and development of

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Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain.Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes.By taking advantage of endogenous DNA repair machinery, these reagents can be …

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Zinc finger nucleases. Zinc finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain (FokI). Zinc finger nucleases were first designed in 2005. The DNA-binding domain can be engineered to target specific DNA sequences while FokI then induces a double strand break.

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Zinc Finger Nuclease (ZFN) technology burst onto the research scene approximately a decade ago, inspiring new visions of genetic modification possibilities. The discovery of the use of ZFNs for deleting genes from and inserting genes into organisms was an …

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Subscribe to the Addgene blog to get all the latest hot plasmid updates! ... TRF2, with the zinc finger domains of TZAP showed that the TZAP zinc finger domains were sufficient to localize TRF2 to telomeres. The plasmids used in this article have been deposited with Addgene and include MYC- and FLAG-tagged TZAP constructs ...

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Zinc fingers of the Cys2 His2 class recognize a wide variety of different DNA sequences and are one of the most abundant DNA-binding motifs found in eukaryotes. The previously determined 2.1 A structure of a complex containing the three zinc fingers from Zif268 has served as a basis for many modeling and design studies, and Zif268 has proved to ...

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help@addgene Wolfe-Lawson ZFN modular assembly kit Description: Zinc-finger nucleases (ZFNs) allow targeted gene inactivation in a wide range of model organisms. We provided an archive of 81 clones containing 27 different zinc-finger modules at finger 1, 2 and

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Plasmid tRNA Synthetase Expression Host pMAH-POLY Polyspecific aminoacyl-tRNA Synthetase Mammalian...protein of interest: you just don't know it yet!The poly-histidine tag often used for protein purification...Green Tc: CCPGCC 50 40-72 Intact Cells Cy3NTAc Poly His 44 35-63 Plasma membrane protein, permeabilized...Lumio Red Tc: CCPGCC 48 38-70 Intact Cells Cy3NTAc Poly His 63 50-90 …

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Inactivation of the phloem-specific Dof zinc finger gene DAG1 affects response to light and integrity of the testa of Arabidopsis seeds. Papi M, et al.Plant Physiol, 2002 Feb. PMID 11842145, Free PMC Article Identification and disruption of an Arabidopsis zinc finger gene controlling seed germination.

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Gene ID: 7593, updated on 5-Jul-2021. Summary Other designations. myeloid zinc finger 1, zinc finger and SCAN domain-containing protein 6, zinc finger protein 42 (myeloid-specific retinoic acid-responsive). GeneRIFs: Gene References Into Functions. MZF1 regulates alpha-globin gene transcription via long-range interactions in erythroid differentiation.

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Addgene is a nonprofit plasmid repository that was founded in 2004 to overcome the obstacles with plasmid sharing. Shortly after its conception, Addgene launched a fruitful collaboration with the Zinc Finger Consortium, which was also in its infancy.

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MTA Ancillary Agreement: Sangamo Zinc-Finger Agreement. Ancillary Agreement for Zinc-Finger Plasmids. Please consult the accompanying implementing letter for definitions of. ORIGINAL MATERIAL, RECIPIENT, and RECIPIENT SCIENTIST regarding this. transfer of the ORIGINAL MATERIAL. By agreeing to this Ancillary Agreement, the RECIPIENT agrees to the.

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Zinc Finger Modules and Binding Sites. (a) A canonical zinc finger module consists of two anti-parallel beta strands and an alpha helix, with a zinc ion coordinated by the conserved cysteine (C) and histidine (H) residues pairs.The numbered amino acid residues at positions −1, +2, +3 and +6, relative to the amino-terminal end of the alpha helix, can form important base-specific contacts in ...

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A zinc finger is a small protein structural motif that is characterized by the coordination of one or more zinc ions (Zn 2+) in order to stabilize the fold.Originally coined to describe the finger-like appearance of a hypothesized structure from Xenopus laevis transcription factor IIIA, the zinc finger name has now come to encompass a wide variety of differing protein structures.

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150 Zinc Finger Consortium - Modular Assembly Kits. (Kit #. 1000000005, 1000000010 ) Zinc …

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（： Zinc-finger nucleases, ZFNs ）,,DNADNA。 DNA。DNA,。

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Zinc Finger Targeter (ZiFiT): An Engineered Zinc Finger/Target Site Design Tool The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters Citation Sander, Jeffry D., Peter Zaback, J. Keith Joung, Daniel F. Voytas, and Drena Dobbs. 2007. Zinc Finger Targeter (ZiFiT): An engineered ...

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Zinc finger protein (ZFP) is a common DNA binding domain found in many transcription factors. It consists of ∼30 amino acids that may recognize three base pairs of DNA. Combining 3–6 ZFP in a molecule, it can recognize and bind to a specific DNA into the genome.

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